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Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
Journal JoVE
Médecine
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Journal JoVE Médecine
Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

DOI:
10.3791/58353-v

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07:45 min

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January 23, 2019

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, , , ,
1Cardiovascular Institute,Allegheny Health Network

Chapitres

  • 00:04Titre
  • 00:40Bedside Collection
  • 01:44Cell Harvest
  • 03:49Cell Purification
  • 05:54Results: Analysis of Endothelial Cells Harvested from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
  • 06:33Conclusion

Summary

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This protocol describes the removal and purification of pulmonary artery endothelial cells (PAECs) that adhere to the balloon tips of Swan-Ganz catheters while they are wedged in the pulmonary artery during right heart catheterization and remain adherent to the deflated balloon following removal from the patient's body.

Tags

Endothelial CellsSwan-Ganz CathetersRight Heart CatheterizationPulmonary Vascular ResearchIntimal ProliferationPulmonary HypertensionCellular BiopsyPulmonary Vascular EndotheliumCell Detachment SolutionCell ScraperBalloon CatheterCatheter Tip

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