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Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis
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Journal JoVE Biologie
Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

DOI:
10.3791/58358-v

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08:16 min

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February 26, 2019

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, , , ,
1Instituto de Investigación Sanitaria, 2Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), 3Departamento de Pediatría, Obstetricia y Ginecología,Universidad de Valencia, 4Servicio de Obstetricia y Ginecología,Hospital Clínico Universitario de Valencia

Chapitres

  • 00:04Titre
  • 00:47Endometrial Tissue Fragment Adenoviral Transfection
  • 02:39Endometrial Implantation
  • 04:08In Vivo Fluorescent Imaging and Quantification
  • 06:05Results: Representative In Vivo Fluorescence Imaging
  • 07:33Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time

Tags

Noninvasive MonitoringLesion SizeHeterologous Mouse ModelEndometriosisAnti-androgenic CompoundsAdeno-mCherryFluorescence MicroscopyOophorectomy

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