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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
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Journal JoVE Médecine
Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

DOI:
10.3791/58399-v

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09:54 min

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November 04, 2018

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, , , , , , , , , ,
1Department of Surgery,Mayo Clinic, 2Midwest Fetal Care Center,Children’s Hospitals and Clinics of Minnesota, 3Department of Surgery,Johns Hopkins, 4Pediatric Surgical Associates

Chapitres

  • 00:04Titre
  • 01:22Laparoscopic Partial Hepatectomy
  • 03:28Hepatocyte Isolation and Transplantation
  • 07:24Results: Representative Lentiviral Vector-mediated Hepatocyte Liver Function Rescue
  • 09:09Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol is intended to describe porcine hepatocyte isolation and ex vivo gene delivery to cure models of metabolic diseases via autologous cell transplantation. Although this particular model enjoys unique advantages that favor successful therapy, the application is a relevant foundation to address additional diseases and indications.

Tags

Lentiviral VectorGene TherapyHepatocytesEx VivoAutologous TransplantationInborn Errors Of MetabolismLiver DiseaseHereditary Tyrosinemia Type 1Surgical ProcedureLaparoscopic TechniqueLiver Resection

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