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1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer,Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering,Imperial College London, 3Department of Bioengineering,Imperial College London, 4Department of Life Sciences,Imperial College London, 5Department of Anaesthetics,Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine,Medical Directorate Joint Force Command
Chapitres
- 00:04Titre
- 00:38Preparation of Hippocampal Organotypic Slices for the Experimental Blast TBI Protocol
- 02:39Submersion and Transport of Tissue Culture Inserts with Hippocampal Organotypic Slices
- 03:44Preparation of the Shock Tube and Hippocampal Organotypic Slice Shock Wave Exposure
- 07:05Results: Typical Shockwave and Resulting Injury in Organotypic Hippocampal Slice
- 08:11Conclusion
Detta dokument beskriver en ny modell av primära blast traumatisk hjärnskada. En tryckluft driven signalledares används för att exponera in vitro- mus Hippocampus slice kulturer till en enda chockvåg. Detta är en enkel och snabb protokoll som genererar en reproducerbar hjärnskada vävnad med ett högt dataflöde.
