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Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
DOI:

11:18 min

January 07, 2019

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Chapitres

  • 00:04Titre
  • 00:52Preparation of Graphite Oxide
  • 02:11GO-deposited Electrode Preparation
  • 03:07Reduction of GO to Electrochemically Reduced Graphene Oxide
  • 04:29Polyaniline Functionalization of the ERGO Electrode
  • 06:09ERGO-PA Electrode Testing at Different pH (Pre-calibration Before Nafion Coating)
  • 06:46Preparation of the Nafion-coated ERGO-PA Electrode
  • 07:22Preparation of L. lactis Culture Medium
  • 08:23Testing of the ERGO-PA-NA pH Response in an L. lactis Fermentation Experiment
  • 09:08Results: Fabrication of ERGO-PA-NA Electrodes to Detect pH Changes During Microbial Fermentation
  • 10:41Conclusion

Summary

Traduction automatique

Here, we report the protocol for the fabrication of a Nafion-coated, polyaniline-functionalized, electrochemically reduced graphene oxide chemiresistive micro pH sensor. This chemiresistor-based, solid-state micro pH sensor can detect pH changes in real-time during a Lactococcus lactis fermentation process.

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