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1Comprehensive Pneumology Center,Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München,Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration,Lund University, 5Wallenberg Center for Molecular Medicine,Lund University, 6Stem Cell Centre,Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado
Chapitres
- 00:04Titre
- 00:49Lung Tissue Inflation
- 02:33Precision-cut Lung Slicing (PCLS) and PCLS Punch Generation
- 04:02Results: Representative Experimental 48 h Human 3D-lung Tissue Culture Readouts
- 04:55Conclusion
Aqui, apresentamos um protocolo para a preparação de fatias de cheias de agarose humano pulmão de precisão de corte de tecido ressecado de pacientes que são adequados para a geração de culturas de tecido de pulmão 3D para doenças de pulmão humano modelo em estudos biológicos e biomédicos.
