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微生物肠道血屏障及肝脏代谢的体内评价方法
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Médecine
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Journal JoVE Médecine
An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products
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微生物肠道血屏障及肝脏代谢的体内评价方法

DOI:
10.3791/58456-v

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14:54 min

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October 20, 2018

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, , , , ,
1Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research,Medical University of Warsaw, 2Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics,Polish Academy of Sciences

Chapitres

  • 00:00Titre
  • 02:17Procedure 1: Insertion of the Line for Intraintestinal Administration
  • 03:57Procedure 2: Inferior Vena Cava Catheterization
  • 06:44Procedure 3: Portal Vein Catheterization
  • 10:46Procedure 4: Portal Vein Blood Sampling
  • 11:22Procedure 5: Inferior Vena Cava Blood Sampling
  • 11:51Procedure 6: Administration of Permeability Marker
  • 12:59Procedure 7: Calculation of Hepatic Clearance
  • 13:20Résultats
  • 14:16Conclusion

Summary

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Traduction automatique

营养物质、微生物代谢物和药物进入血液循环的途径由肠道血屏障 (GBB) 控制。我们描述了一种直接测量体内 GBB 通透性的方法, 与常用的间接方法相比, 它几乎不受肝肾功能的影响。

Tags

Gut-blood BarrierGut MicrobiotaPortal Blood SamplingHepatic MetabolismIntestinal PermeabilityIn Vivo MethodMarker AdministrationFastingGeneral AnesthesiaPortal VeinInferior Vena CavaGastrointestinal Tract

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