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1Department of Child Health,University of Arizona College of Medicine, 2Department of Biological Sciences,University of Bath, 3BARROW Neurological Institute at Phoenix Children’s Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences,University of Arizona College of Medicine
Chapitres
- 00:04Titre
- 00:44Cryoprotection of Perfused Brains and Brain Freezing
- 03:18Preparing the Megabrain for Sectioning on the Cryostat
- 04:14Cryosectioning the Megabrain
- 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
- 05:54Conclusion
Aqui, apresentamos um protocolo para congelar e secção de tecido cerebral de vários animais como uma alternativa economiza tempo de processamento único cérebro. Isto reduz a variabilidade de coloração durante a imuno-histoquímica e reduz o tempo cryosectioning e imagens.
