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Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
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Chapitres
- 00:04Titre
- 00:50Mouse Preparation and Direct Intrathecal Adeno-associated Virus (AAV) Delivery
- 03:16Immunohistochemical Tissue Preparation
- 04:46Immunohistochemistry
- 06:27Results: Representative AAV Transduction After Direct Intrathecal Delivery
- 07:52Conclusion
Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.
