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1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology,Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology,Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control,Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests,Fujian Agriculture and Forestry University
Chapitres
- 00:04Titre
- 01:01Ligation-independent Cloning (LIC)
- 02:38E. coli Transformation and Protein Expression
- 04:58Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification
- 06:12Protein Crystallization, Crystal Mounting, and Structure Determination
- 09:56Results: Representative N-terminal Domain (NTD) Crystal Structure Determination
- 10:53Conclusion
