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In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

DOI:
10.3791/58570-v

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09:49 min

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November 20, 2018

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, ,
1Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Chapitres

  • 00:04Titre
  • 00:45Continuous Mode ThT Aggregation Assay on a Multi-Mode Microplate Reader
  • 06:43Results: In Vitro Assay to Characterize Tau Protein Aggregation
  • 08:50Conclusion

Summary

Traduction automatique

Traduction automatique

The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.

Tags

Tau ProteinAlzheimer’s DiseaseTauopathiesAggregation AssayDrug ScreeningNucleation-dependent PolymerizationRecombinant TauFluorescenceSEC-MALSHeparinThioflavin-TMicroplate Reader

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