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選択したプロウイルス統合サイトの HIV-1 感染の Jurkat レポーターのモデルを生成する CRISPR ベース Cas9 ゲノム工学
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Chapitres
- 00:04Titre
- 00:42Choice of Targeted Locus, Guide RNA (gRNA), and Targeting Vector Design
- 03:08CRISPR-Cas9-based Targeting of Jurkat Cells
- 04:36Generation of Clonal Lines and Screening for Correct Targeting
- 07:50Screening of Single-cell Clones by Flow Cytometry and PCR
- 11:46Results: Screening and Analysis of Single-cell Clones for Correct Reporter Integration
- 13:16Conclusion
