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Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

DOI:
10.3791/58595-v

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08:13 min

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January 20, 2019

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, , , , , , , ,
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Chapitres

  • 00:04Titre
  • 00:58Preparation of ELISpot-plates and Cell-stimulation
  • 02:09Blood Sample Processing
  • 03:44Plate Preparation and Cell-plating
  • 04:47Detection of Interferon-gamma Positive Spots
  • 06:34Results: ELISpot Assay Used to Characterize T-cell Responses in Guinea Pigs
  • 07:15Conclusion

Summary

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The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.

Tags

Keywords: ELISpot AssayT Cell ResponsesGuinea Pig ModelVaccinationImmunotherapeuticsHumoral ImmunityPeripheral BloodKineticsCapture Anti-guinea Pig Interferon Gamma Antibody VE4Density Gradient MediumPBMCs

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