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Etablering av dual Humanisert TK-NOG Mouse modell for HIV-assosiert Liver patogenesen
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Immunologie et infection
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Journal JoVE Immunologie et infection
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Etablering av dual Humanisert TK-NOG Mouse modell for HIV-assosiert Liver patogenesen

DOI:
10.3791/58645-v

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00:10 min

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September 11, 2019

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, , , ,
1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, 2Department of Pathology and Microbiology,University of Nebraska Medical Center

Chapitres

  • 00:04Titre
  • 00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
  • 04:25Preparation of Human Hepatocytes for Transplantation
  • 05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
  • 06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
  • 07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
  • 09:25Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokollen gir en pålitelig metode for å etablere humanisert mus med både humant immunsystem og leverceller. Dual rekonstituert immunodeficient mus oppnådd via intrasplenic injeksjon av menneskelig HEPATOCYTTER og CD34+ blodkreft stamceller er mottakelige for humant immunsviktvirus-1 infeksjon og recapitulate leverskader som observert i HIV-infiserte pasienter.

Tags

HIVLiver PathogenesisHumanized Mouse ModelHematopoietic Stem CellsHepatocytesUmbilical Cord BloodCD34 Positive CellsCell SeparationMagnetic Activated Cell Sorting (MACS)Cell CountingCell CultureTransplantation

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