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qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
Journal JoVE
Génétique
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Journal JoVE Génétique
qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
DOI:

07:58 min

March 06, 2019

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Chapitres

  • 00:04Titre
  • 01:45Preparation and Plating Out of DNA
  • 02:25Preparation of the Master Mix
  • 03:36qPCR Assay
  • 04:07Post-run Analysis
  • 05:19Export of Results and Copy Number Calculations
  • 06:39Results: qKAT Used to Copy Number Type KIR Genes
  • 06:59Conclusion

Summary

Traduction automatique

Quantitative killer cell immunoglobulin-like receptor (KIR) semi-automated typing (qKAT) is a simple, high-throughput, and cost-effective method to copy number type KIR genes for their application in population and disease association studies.

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