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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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Médecine
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Journal JoVE Médecine
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

DOI:
10.3791/58660-v

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09:36 min

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February 05, 2019

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, , , , , ,
1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University

Chapitres

  • 00:04Titre
  • 01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
  • 03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
  • 05:00In Vitro RAW264.7 Macrophages Transfection
  • 06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
  • 08:51Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

Tags

PolyethyleneimineIron Oxide NanoparticlesSPIONMacrophageDeliverySiRNAIn VitroIn VivoAllylic AcidDimercapto Acrylic AcidTriethylaminePHNanoparticle SynthesisCoatingInflammationCancer

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