Journal
/
Recherche en cancérologie
/
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

DOI:
10.3791/58672-v

•

10:44 min

•

August 09, 2019

•

, , , , , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research,National Cancer Institute, NIH

Chapitres

  • 00:04Titre
  • 00:57Preparation of OP9/DLL1 Cells for Co-culture with iPSC
  • 02:08In Vitro Differentiation of iPSC into Immature T Cells
  • 05:003D Thymic Organ Culture to Generate iTE
  • 08:00Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
  • 09:46Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

Tags

3D Thymic CultureInduced Pluripotent Stem CellsTumor Antigen-specific T CellsThymic EmigrantsOP9/DLL1 CellsTrypsinizationCell SuspensionCell CultureT Cell DifferentiationIn Vivo T Cell Generation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

A Mouse Model of Fatigue Induced by Peripheral Irradiation

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Read Article