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En tredimensionel Thymisk kultur system til at generere murine induceret pluripotente stamcelle-afledte tumor antigen-specifikke Thymisk emigranter
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
DOI:

10:44 min

August 09, 2019

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Chapitres

  • 00:04Titre
  • 00:57Preparation of OP9/DLL1 Cells for Co-culture with iPSC
  • 02:08In Vitro Differentiation of iPSC into Immature T Cells
  • 05:003D Thymic Organ Culture to Generate iTE
  • 08:00Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
  • 09:46Conclusion

Summary

Traduction automatique

Denne artikel beskriver en ny metode til at generere tumor antigen-specifikke induceret pluripotente stamcelle-afledte thymisk emigranter (iTE) af en tredimensionel (3D) thymisk kultur system. iTE er en homogen delmængde af T-celler tæt relateret til naive T-celler med kapacitet til spredning, hukommelse dannelse, og tumor suppression.

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