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Developmental Biology

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JoVE Journal Developmental Biology
Mikrofluid analyse til vurdering af leukocyt adhæsion til menneskelige induceret pluripotente stamceller-afledt endotelceller (hiPSC-ECs)
 
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Mikrofluid analyse til vurdering af leukocyt adhæsion til menneskelige induceret pluripotente stamceller-afledt endotelceller (hiPSC-ECs)

Article DOI: 10.3791/58678-v • 06:47 min • November 26th, 2018
November 26th, 2018 •
1, 1, 1,2, 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

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Denne trinvise protokol indeholder en detaljeret beskrivelse af eksperimentel opsætning og dataanalyse til vurdering af inflammatoriske respons i hiPSC-ECs og analyse af leukocyt adhæsion under fysiologiske flow.

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Udviklingsmæssige biologi sag 141 menneskelige induceret pluripotente stamceller-afledt endothelial celler (hiPSC-ECs) inflammatoriske respons leukocytter flow vedhæftning assay mikrofluidik live imaging
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