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Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
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Biologie
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Journal JoVE Biologie
Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

DOI:
10.3791/58683-v

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06:19 min

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November 17, 2018

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, , , , , ,
1Translational Medicine Program,The Hospital for Sick Children, 2Department of Laboratory Medicine and Pathobiology,University of Toronto, 3Neurosciences & Mental Health Program,The Hospital for Sick Children, 4Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine,Yeungnam University, 5Banting and Best Diabetes Centre,University of Toronto

Chapitres

  • 00:04Titre
  • 00:59Whole-mount Staining of White Adipose Tissue
  • 03:51Results: Analysis of White Adipose Tissue Preserved Using Whole-mount Staining
  • 05:37Conclusion

Summary

Traduction automatique

Traduction automatique

The focus of the present study is to demonstrate the whole-mount immunostaining and visualization technique as an ideal method for 3D imaging of adipose tissue architecture and cellular component.

Tags

3D VisualizationWhole-mount StainingMetabolic ConditionsFastingObesityImmunohistochemistryFixationAntibody StainingBlockingPrimary AntibodiesSecondary AntibodiesNeutral Lipid Staining

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