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Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom
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1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System
Chapitres
- 00:04Titre
- 00:57Monoplex Tyramide-Based Immunofluorescence
- 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
- 04:36Data Analysis
- 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
- 07:10Conclusion
Her beskriver vi en protokol for multiplex fluorescerende immunhistokemisk farvning og imaging for simultan lokalisering af flere kræft-associerede antigener i lymfom. Denne protokol kan udvides til en colocalization analyse af biomarkører inden for alle væv sektioner.
