Journal
/
Biologie
/
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

DOI:
10.3791/58748-v

•

09:23 min

•

November 28, 2018

•

, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Chapitres

  • 00:04Titre
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes protocols to assess the effect of fluorescent proteins on the aggregation and toxicity of misfolded polyglutamine expansion for the rapid evaluation of a newly uncharacterized fluorescent protein in the context of fluorescent reporters.

Tags

Keywords: Fluorescent ProteinsFusion PartnersPolyglutamine ToxicityYeastHTT Exon One25Q72QMonomeric Superfolder GFPGAL1 PromoterPolyQ FusionsOptical Density

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/58748?article_type=v

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
check_url/fr/58748?article_type=v

Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System
check_url/fr/58748?article_type=v

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
check_url/fr/58748?article_type=v

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
check_url/fr/58748?article_type=v

Growth Assays to Assess Polyglutamine Toxicity in Yeast
check_url/fr/58748?article_type=v

Identification of Protein Interacting Partners Using Tandem Affinity Purification
check_url/fr/58748?article_type=v

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
check_url/fr/58748?article_type=v

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
check_url/fr/58748?article_type=v

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
check_url/fr/58748?article_type=v

Comparing the Affinity of GTPase-binding Proteins using Competition Assays

Read Article