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Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær
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Journal JoVE Biologie
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær

DOI:
10.3791/58748-v

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09:23 min

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November 28, 2018

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, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Chapitres

  • 00:04Titre
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

Traduction automatique

Traduction automatique

Denne artikel beskriver protokoller for at vurdere effekten af fluorescerende proteiner på en sammenlægning og toksicitet af fejlfoldede polyglutamin udvidelse til hurtig evaluering af en nyligt uncharacterized fluorescerende proteiner inden for rammerne af fluorescerende journalister.

Tags

Keywords: Fluorescent ProteinsFusion PartnersPolyglutamine ToxicityYeastHTT Exon One25Q72QMonomeric Superfolder GFPGAL1 PromoterPolyQ FusionsOptical Density

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