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Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

DOI:
10.3791/58756-v

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10:19 min

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January 21, 2019

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,
1UKE Microscopy Imaging Facility,University Medical Center Hamburg-Eppendorf

Chapitres

  • 00:04Titre
  • 01:15Cell Preparation
  • 04:12Live Cell Imaging
  • 06:50Image Post-processing in ImageJ
  • 08:14Results: Cell Spreading and Adhesion Formation using SpinningDisk-TIRF Microscopy
  • 09:13Conclusion

Summary

Traduction automatique

Traduction automatique

An advanced microscope that permit fast and high-resolution imaging of both, the isolated plasma membrane and the surrounding intracellular volume, will be presented. The integration of spinning disk and total internal reflection fluorescence microscopy in one setup allows live imaging experiments at high acquisition rates up to 3.5 s per image stack.

Tags

Keywords: Spinning DiskTIRF MicroscopyCytoskeletal NetworkPlasma MembraneLive ImagingCell BiologyMicrobiologyFluorescence MicroscopyRFP LivactYFP VinculinFibronectinMulti-fluorescent Beads

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