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Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Neurosciences
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Journal JoVE Neurosciences
Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

DOI:
10.3791/58799-v

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05:45 min

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April 26, 2019

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, , , ,
1Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Chapitres

  • 00:04Titre
  • 00:40Rectal Suction Biopsy (RSB) Section Preparation
  • 01:52Antigen Retrieval and Tissue Staining
  • 04:25Results: Representative RSB Immunohistochemical Analysis
  • 05:09Conclusion

Summary

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This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.

Tags

Hirschsprung’s DiseaseImmunostainingRectal Suction BiopsyCalretininS100 ProteinProtein Gene Product 9.5DiagnosisSensitivitySpecificityParaformaldehydeDehydrationParaffin EmbeddingMicrotomeAntigen RetrievalHydrogen PeroxideBovine Serum AlbuminPrimary AntibodyPolymer EnhancerSecondary Antibody

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