Journal
/
Bio-ingénierie
/
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

DOI:
10.3791/58830-v

•

06:15 min

•

January 30, 2019

•

,
1Nanotechnology Characterization Lab., Cancer Research Technology Program,Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute

Chapitres

  • 00:04Titre
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

Traduction automatique

Traduction automatique

Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles.

Tags

Keywords: EndotoxinNano-formulationsLimulus Amoebocyte Lysate (LAL) AssaysCalibration StandardControl Standard EndotoxinLAL Grade WaterQuality ControlInstrument SettingsTest IDData GroupHardwareLAL MethodSerial NumberSystem IDSerial PortSample IDNegative ControlStandard CurveTest SamplesInhibition Enhancement ControlDilution

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/58830?article_type=v

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology
check_url/fr/58830?article_type=v

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
check_url/fr/58830?article_type=v

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
check_url/fr/58830?article_type=v

Bacterial Detection & Identification Using Electrochemical Sensors
check_url/fr/58830?article_type=v

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays
check_url/fr/58830?article_type=v

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
check_url/fr/58830?article_type=v

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
check_url/fr/58830?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/fr/58830?article_type=v

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
check_url/fr/58830?article_type=v

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Read Article