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1Department of Biological Sciences,California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology,California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry,California Polytechnic State University, 4Department of Chemical and Biological Engineering,Northwestern University, 5Chemistry of Life Processes Institute,Northwestern University, 6Center for Synthetic Biology,Northwestern University, 7Interdisciplinary Biological Sciences Program,Northwestern University
Chapitres
- 00:04Titre
- 00:50Cell Growth
- 03:30Crude Cell Extract Preparation
- 05:30Cell-Free Protein Synthesis Batch Format Reactions
- 06:25Quantification of the Reporter Protein, [sfGFP]
- 07:24Results: Modification and Optimization of the Cell-Free Platform Conditions
- 09:01Conclusion
Detta protokoll uppgifter om åtgärder, kostnader och utrustning krävs för att generera E. coli-baserade cell extrakt och genomföra in vitro- proteinsyntes reaktioner inom 4 dagar eller mindre. För att utnyttja flexibiliteten i denna plattform för bred applikationer, diskutera vi reaktionsbetingelser som kan anpassas och optimeras.
