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At udnytte Live Imaging til Track kerner under Myoblast differentiering og Fusion
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Journal JoVE Biologie
Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

At udnytte Live Imaging til Track kerner under Myoblast differentiering og Fusion

DOI:
10.3791/58888-v

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09:03 min

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April 13, 2019

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, , , , , ,
1Chromatin Dynamics Unit,Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3Department of Electronics, Information and Bioengineering,Politecnico di Milano

Chapitres

  • 00:00Titre
  • 00:43Primary Myoblast Isolation
  • 03:30Myoblast Proliferation and Differentiation
  • 06:25Results: Representative Myoblast Nucleus Differentiation and Tracking Analyses
  • 08:14Conclusion

Summary

Traduction automatique

Traduction automatique

Skeletmuskulatur differentiering er en yderst dynamisk proces, som især afhængig af nukleare positionering. Her, beskriver vi en metode til at spore kerner bevægelser af levende celle imaging under myoblast differentiering og myotube dannelse og udføre en kvantitativ karakterisering af kerner dynamics af uddrager information af automatisk sporing.

Tags

Live Cell ImagingMyoblast DifferentiationMyofiber FormationNuclear PositioningPrimary Cell IsolationCell TrackingMuscle Cell IsolationSatellite Cell Isolation

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