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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
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Journal JoVE Médecine
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

DOI:
10.3791/58891-v

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08:18 min

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January 07, 2019

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, ,
1Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS,Hospital Clínic de Barcelona

Chapitres

  • 00:04Titre
  • 01:05NBD-cholesterol Preparation, Cell Culture, and Seeding
  • 02:20Apolipoprotein B Depleted Serum (ABDS) Preparation
  • 03:14NBD-cholesterol Cell Loading and Incubation with Cholesterol Acceptors
  • 04:29Fluorescent Signal Capture
  • 05:35Results: Analysis of Cholesterol Efflux Assay
  • 07:24Conclusion

Summary

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Traduction automatique

Measurement of in vitro cholesterol efflux capacity to serum or plasma in macrophage cell models is a promising tool as a biomarker for atherosclerosis. In the present study, we optimize and standardize a fluorescent NBD-cholesterol efflux method and develop a high-throughput analysis using 96-well plates.

Tags

Cholesterol EffluxNBD CholesterolTHP-1 CellsPEG PrecipitationCardiovascular RiskCell-based AssayHigh-throughput ScreeningLipid MetabolismMacrophagesStem Cells

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