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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
DOI:

10:14 min

May 12, 2019

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Chapitres

  • 00:04Titre
  • 01:09Brain Surface Scan, Slicing, and Multi-electrode Array Recording
  • 05:23MRI Image Processing to Stripe Cortical Surfaces
  • 06:32Preprocessing for 3D Scan Data and Coregistration of the MRI Surface and the 3D Scan Surface
  • 08:18Results: Overlapping Accuracy Between MRIs and 3D Scans
  • 09:17Conclusion

Summary

Traduction automatique

This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).

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