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Journal / Genetics / 非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰…
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JoVE Journal Genetics
非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰
 
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非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰

Article DOI: 10.3791/58933-v • 11:16 min • February 15th, 2019
February 15th, 2019 •
1, 1, 1, 1, 1, 1, 1,2,3, 1,2
1Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Research Center, 2Department of Medicine, University of Washington, 3Department of Pathology, University of Washington

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该方案的目标是从启动的骨髓中分离出非人类灵长类动物 cd34+细胞, 用慢病毒载体对这些细胞进行基因修饰, 并准备一种产品, 用于注入自体宿主。协议的总长度约为48小时。

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遗传学 第144期 造血干细胞和祖细胞 基因治疗 造血 非人类灵长类模型 细胞分离 慢病毒载体
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