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An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
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Chapitres
- 00:04Titre
- 00:31Preparation of 3% Sucrose Containing 1/2 Murashige-Skoog Medium Solution
- 01:12Induction of Clustered Stomata by Sucrose-containing Medium Solution Immersion Treatment
- 02:30Microscopic Observation of Clustered Stomata
- 03:15Stomata Clustering Induced in A. thaliana Seedlings
- 04:06Conclusion
The goal of this protocol is to demonstrate how to induce clustered stomata in cotyledons of Arabidopsis thaliana seedlings by immersion treatment with a sugar-containing medium solution and how to observe intracellular structures such as chloroplasts and microtubules in the clustered guard cells using confocal laser microscopy.
