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En rustfri protokol for høj kvalitet RNA isolering fra Laser fange Microdissected Purkinje celler i den menneskelige Post Mortem lillehjernen
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1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University
Chapitres
- 00:04Titre
- 00:33Prepare Cryostat for Tissue Sectioning
- 01:51Sectioning Tissue
- 03:50Fixing Tissue/Stain-less Visualization
- 04:47Laser Capture Microdissection of Purkinje Cells
- 06:09Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
- 07:59Conclusion
Denne protokol bruger en plet-fri tilgang til at visualisere og isolere Purkinje celler i frisk frosset væv fra menneskelige post mortem lillehjernen via laser fange microdissection. Formålet med denne protokol er at generere tilstrækkelige mængder af høj kvalitet RNA til RNA-sekvens.
