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Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

DOI:
10.3791/58957-v

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08:20 min

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May 21, 2019

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, , , , , , , , , ,
1Department of Biomedical Engineering, Knight Cancer Institute,Oregon Health and Science University, 2Quantitative Imaging Systems LLC

Chapitres

  • 00:04Titre
  • 00:47Printing Microenvironment Microarrays (MEMAs) Using an Array Printing Robot
  • 01:37Creation of Ligand Treatment Plates
  • 02:28Culturing Cells on MEMAs
  • 04:23Fixing, Staining, and Imaging MEMAs
  • 06:14Results: Cell Cycle Profiles, Cell Number and EdU Incorporation
  • 07:44Conclusion

Summary

Traduction automatique

Traduction automatique

The purpose of the method presented here is to show how microenvironment microarrays (MEMA) can be fabricated and used to interrogate the impact of thousands of simple combinatorial microenvironments on the phenotype of cultured cells.

Tags

Keywords: MicroarrayMicroenvironmentTumorPhenotypeMEMA PlatformExtracellular MatrixMicroenvironment MicroarraysCollagenLigandCell CultureBlocking BufferPBSMCF7 Cells

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