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荧光活化细胞分选建立纯化的 Gabaep 或谷胱甘肽神经元的原代细胞培养

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

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荧光活化细胞分选建立纯化的 Gabaep 或谷胱甘肽神经元的原代细胞培养

DOI:

10.3791/58974-v

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10:57 min

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June 06, 2019

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Paul Turko, Keenan Groberman, Toralf Kaiser, Yuchio Yanagawa, Imre Vida

¹Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, ²Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, ³Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Chapitres

00:04Titre
00:47Coating Glass Coverslips
02:22Dissociation of Hippocampal and Cortical Tissue
07:13Cell Sorting and Culturing of Purified GABAergic or Glutamatergic Neurons
09:19Results: Cell Culture of Purified Glutamatergic or GABAergic Neurons
10:26Conclusion

Summary

Traduction automatique

该协议描述了一种基于细胞分选的方法, 用于从产后小鼠或大鼠的新皮层和海马中纯化和培养荧光 gabaep 或谷氨酸神经元。

Tags

Primary Cell Culture GABAergic Neurons Glutamatergic Neurons Fluorescence-activated Cell Sorting Neuronal Physiology Synapses Neuronal Networks PLL Coating Cell Culture Buffer BSA Papain Filter Sterilization

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