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Designing Porous Silicon Films as Carriers of Nerve Growth Factor
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

DOI:
10.3791/58982-v

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10:12 min

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January 25, 2019

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, , ,
1Department of Biotechnology and Food Engineering,Technion – Israel Institute of Technology, 2Faculty of Engineering,Bar-Ilan University, 3Bar-Ilan Institute of Nanotechnologies and Advanced Materials, 4Russell Berrie Nanotechnology Institute,Technion – Israel Institute of Technology

Chapitres

  • 00:04Titre
  • 00:57Oxidized Porous Silicon (PSiO2) Carrier Fabrication
  • 02:10Nerve Growth Factor (NGF) Loading and Quantification and In Vitro NGF Release from PSiO2 Carriers
  • 04:42Cell Viability and Growth in the Presence of NGF-Loaded PSiO2 Carriers
  • 06:00Cell Differentiation Analysis
  • 07:27Results: Representative Effects of NGF-Loaded PSiO2 Carriers on Neuronal Growth and Development
  • 08:58Conclusion

Summary

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Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.

Tags

Keywords: Porous SiliconNerve Growth FactorNGFCarrierBioactive ProteinReservoir ImplantFabrication ProcessElectrochemical EtchingThermal OxidationLoading SolutionELISA

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