Journal
/
Neurosciences
/
Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

DOI:
10.3791/59036-v

•

07:02 min

•

January 19, 2019

•

, , ,
1Department of Neurology,University of Michigan School of Medicine, 2Neuroscience Graduate Program,University of Michigan School of Medicine

Chapitres

  • 00:04Titre
  • 01:06Transfection of Rat Cortical Neurons
  • 02:22Imaging
  • 03:09Image Processing and Scoring Cell Death
  • 04:35Performing Cox Proportional Hazards Analysis and Visualizing Results
  • 05:19Results: Assessing Neuronal Survival on a Single-cell Basis
  • 06:16Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to monitor survival on a single-cell basis and identify variables that significantly predict cell death.

Tags

Neuronal SurvivalLongitudinal Fluorescence MicroscopySingle-cell MonitoringCytotoxicity AssaysNeurodegenerative DiseasesCell DeathTransfection EfficiencyMultichannel PipetCortical NeuronsNBKY SolutionConditioned MediaNBC SolutionFluorescent MicroscopeFiduciary MarkX And Y CoordinatesFluorescent Label

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59036?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/fr/59036?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/fr/59036?article_type=v

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
check_url/fr/59036?article_type=v

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis
check_url/fr/59036?article_type=v

Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats
check_url/fr/59036?article_type=v

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
check_url/fr/59036?article_type=v

Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures
check_url/fr/59036?article_type=v

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy
check_url/fr/59036?article_type=v

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
check_url/fr/59036?article_type=v

Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses

Read Article