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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

DOI:
10.3791/59072-v

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06:32 min

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July 02, 2019

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, ,
1Department of Pharmacology and Toxicology,University of Mississippi Medical Center

Chapitres

  • 00:04Titre
  • 00:38Equipment Preparation
  • 03:13Middle Cerebral Artery (MCA) Isolation and Cannulation
  • 05:22Results: Representative MCA Membrane Potential (Vm) Analyses
  • 06:09Conclusion

Summary

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The primary goal of this article is to provide details of how to record membrane potential (Vm) from the middle cerebral artery using the microelectrode impalement method. The cannulated middle cerebral artery is equilibrated to gain myogenic tone, and the vessel wall is impaled using high resistance microelectrodes.

Tags

MicroelectrodeMembrane PotentialMiddle Cerebral ArteryVascular ToneBlood FlowElectrometer AmplifierBorosilicate Glass MicroelectrodesPotassium ChloridePhysiological Salt SolutionVascular Smooth MuscleCannulationMyograph

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