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To-fartøj okklusion musemodel af Cerebral iskæmi-reperfusion
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Médecine
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Journal JoVE Médecine
Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

To-fartøj okklusion musemodel af Cerebral iskæmi-reperfusion

DOI:
10.3791/59078-v

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07:34 min

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March 01, 2019

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, ,
1Research Center of Translational Imaging, College of Medicine,Taipei Medical University, 2Radiogenomic Research Center,Taipei Medical University Hospital, Taipei Medical University, 3Department of Radiology, School of Medicine, College of Medicine,Taipei Medical University, 4Department of Medical Imaging, Taipei Medical University Hospital,Taipei Medical University, 5Department of Medical Research, Taipei Medical University Hospital,Taipei Medical University, 6Department of Surgery, School of Medicine, College of Medicine,Taipei Medical University, 7Division of General Surgery, Department of Surgery,Taipei Medical University Hospital

Chapitres

  • 00:04Titre
  • 00:58Middle Cerebral Artery Occlusion (MCAO)/Reperfusion Model
  • 03:48Staining with 2,3,5-triphenyltetrazolium Chloride
  • 05:03Infarct Size Measurement and Statistical Analysis
  • 06:09Results: Analysis of Mouse Brain after Ischemia-induced Infarct
  • 06:52Conclusion

Summary

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Traduction automatique

En musemodel af cerebral iskæmi-reperfusion er etableret for at undersøge Patofysiologi af slagtilfælde. Vi distalt ligate lige midt cerebral arterie og højre fælles halspulsåren og genoprette blodtilførslen efter 10 eller 40 min for iskæmi.

Tags

Keywords: Two-vessel OcclusionMouse ModelCerebral Ischemia-reperfusionInfarct VolumeBrain Infiltrating Immune CellsBehavioral DeficitMCAO ReperfusionCraniotomyDistal MCACommon Carotid ArteryZygomatic And Squamosal BoneMiddle Cerebral ArteryAneurism ClipIschemiaReperfusion

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