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En høj overførselshastighed Assay for forudsigelse af kemiske toksicitet af automatiseret fænotypiske profilering af Caenorhabditis elegans
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1Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, China, 2College of Computer Science and Technology,Qilu University of Technology(Shandong Academy of Sciences), China, 3Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, China, 4Beijing Key Laboratory of Environmental Toxicology, School of Public Health,Capital Medical University, China, 5School of Life Sciences,La Trobe University, Australia
Chapitres
- 00:04Titre
- 01:38Chemical Preparation
- 02:28Worm Preparation
- 04:55Chemical Treatment and Experiment Video Processing
- 06:47Results: Mortality and Motility After Chemical Treatment
- 08:10Conclusion
En kvantitativ metode er blevet udviklet for at identificere og forudse kemikalier akutte toksicitet af automatisk analysere fænotypiske profilering af Caenorhabditis elegans. Denne protokol beskriver, hvordan du behandler orme med kemikalier i en 384-godt plade, fange videoer og kvantificere toksikologiske relaterede fænotyper.
