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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
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Journal JoVE Environnement
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

DOI:
10.3791/59106-v

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11:08 min

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January 07, 2019

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, ,
1Department of Biosystems Engineering,Auburn University

Chapitres

  • 00:04Titre
  • 00:42Setup of Bubble Column Photobioreactors
  • 02:13Cultivation of Microalgae in Bubble Column Photobioreactors
  • 04:03Harvest and Freeze Drying of Microalgal Biomass
  • 05:28Lipid Extraction Using a Modified Folch Method
  • 07:07Neutral Lipid Assay Using a Microplate Method (Adapted from Higgins et al. 201422)
  • 09:01Results: Auxenochlorella protothecoides Growth Results and Chlorella sorokiniana Neutral Lipid Data
  • 10:35Conclusion

Summary

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Here, we present a protocol to construct lab-scale bubble column photobioreactors and use them to culture microalgae. It also provides a method for the determination of the culture growth rate and neutral lipid content.

Tags

Keywords: MicroalgaeBubble Column PhotobioreactorAlgae BiofuelsWastewater TreatmentAlgae BiologyReactor SystemO-ringsCheck ValvesAir FiltersCentrifugationAlgae ConcentrationPhotobioreactors

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