Journal
/
Biochimie
/
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

DOI:
10.3791/59112-v

•

06:06 min

•

June 21, 2019

•

, ,
1Department of Molecular Biology and Biochemistry,Simon Fraser University

Chapitres

  • 00:04Titre
  • 00:41Preparing and Loading Denaturing Gels
  • 02:30Post Gel Staining
  • 03:17Imaging Mango Tagged RNAs in Gel
  • 04:01Results: Post-gel Staining Method to Image RNAs Tagged with RNA Mango Aptamers
  • 05:37Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.

Tags

Mango-tagged RNAPolyacrylamide GelsPoststaining MethodFluorescent VisualizationRNA PurificationRNA TrackingRNA VisualizationDenaturing GelGel ElectrophoresisRNA SamplesGel Staining SolutionGel Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

A Colorimetric Method for Measuring Iron Content in Plants

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Read Article