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Biologiske prøveforberedelse højtryks frysebehandling, mikrobølgeovn-assisteret kontrastforbedring og Minimal harpiks indlejring for volumen Imaging
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Biologie
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Journal JoVE Biologie
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Biologiske prøveforberedelse højtryks frysebehandling, mikrobølgeovn-assisteret kontrastforbedring og Minimal harpiks indlejring for volumen Imaging

DOI:
10.3791/59156-v

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07:33 min

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March 19, 2019

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, ,
1Electron Microscopy Core Unit,Max Planck Institute of Experimental Medicine, 2Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Chapitres

  • 00:04Titre
  • 00:43High-pressure Freezing
  • 01:32Freeze Substitution
  • 02:39Microwave-assisted Processing
  • 04:10Minimal Resin Embedding
  • 04:43Preparation for FIB-SEM (Focused Ion Beam Scanning Electron Microscope)
  • 05:36“
  • 06:55Conclusion

Summary

Traduction automatique

Traduction automatique

Her præsenterer vi en protokol til at kombinere to prøve behandling teknikker, højtryks frysning og mikrobølgeovn-assisteret prøve behandling, efterfulgt af minimal harpiks indlejring for at opnå data med en fokuseret ion beam scanning elektron mikroskop (FIB-SEM). Dette er påvist ved hjælp af en mus tibial nerve prøve og Caenorhabditis elegans.

Tags

Keywords: High-pressure FreezingMicrowave-assisted Contrast EnhancementMinimal Resin EmbeddingVolume ImagingFocused Ion Beam Scanning Electron MicroscopeUltrastructure PreservationFreeze SubstitutionOsmium TetroxideUranyl AcetateSample PreparationNervus Tibialis

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