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Kombinerede nukleotid og Protein ekstraktioner i Caenorhabditis elegans
Journal JoVE
Biologie
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Journal JoVE Biologie
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Kombinerede nukleotid og Protein ekstraktioner i Caenorhabditis elegans

DOI:
10.3791/59178-v

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10:37 min

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March 17, 2019

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, , ,
1Department of Molecular Biology, Cell Biology and Biochemistry,Brown University

Chapitres

  • 00:04Titre
  • 00:33Sample Collection
  • 01:31Nucleotide and Protein Isolation
  • 07:01Evaluating mRNA with RT-qPCR
  • 08:12Results: Extractions of Different Macromolecules from the Same C. elegans Sample
  • 09:40Conclusion

Summary

Traduction automatique

Traduction automatique

Vi præsenterer her, en protokol til isolering af RNA, DNA og protein fra den samme prøve, i et forsøg på at reducere variation, forbedre reproducerbarhed og lette fortolkninger.

Tags

Caenorhabditis ElegansNucleotide ExtractionProtein ExtractionMRNAMacromoleculesSample VariabilityReproducibilitySample CollectionCross-sectional AnalysisWormsM9 BufferGTCP ReagentChloroformRNA IsolationIsopropanolEthanolDNA IsolationProtein Isolation

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