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Immunologie et infection
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A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

DOI:
10.3791/59201-v

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05:28 min

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August 15, 2019

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, , , ,
1Department of Bioengineering and Institute of Nanoscience and Technology,Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases,Yale University School of Medicine

Chapitres

  • 00:04Titre
  • 00:28Mouse Positioning
  • 01:23Intranasal Delivery
  • 03:28Results: Distribution of Fluorescently Labeled Peptide and siRNA after Intranasal Administration
  • 04:52Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in the central nervous system.

Tags

Keywords: Positioning DeviceIntranasal DeliveryCentral Nervous SystemSiRNAMiceAnesthesiaIntranasal InoculationRVG9R SiRNA ComplexMicropipetteReproducibility

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