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1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles
Chapitres
- 00:04Titre
- 00:40Vector Design and Generation
- 01:52Virus Generation
- 04:24Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
- 07:07dCas9 and gRNA Transduction, Selection, and Clone Creation
- 10:18Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
- 12:08Conclusion
Tradisjonelle cDNA-baserte overuttrykte teknikker har et begrenset bruksområde for overuttrykte lang noncoding RNAs på grunn av skjemaene for flere skjøte med mulig funksjonalitet. Denne anmeldelsen rapporterer en protokoll bruker CRISPR teknologi for å overexpress flere skjøte-varianter av en lang noncoding.
