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Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
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Journal JoVE Recherche en cancérologie
Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis

DOI:
10.3791/59236-v

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07:05 min

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May 12, 2019

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, ,
1Department of Biomedical Sciences, Faculty of Health and Medical Sciences,University of Copenhagen, 2Novo Nordisk Foundation Center of Basic Metabolic Research, Faculty of Health and Medical Sciences,University of Copenhagen

Chapitres

  • 00:04Titre
  • 00:325-Fluorouracil (5-FU) Mucositis Induction
  • 01:095-Bromo-2 -Deoxyuridine (BrdU) Quantification and Tissue Collectio
  • 02:42BrdU Immunoreactive Cell Area Measurement
  • 04:23Crypt Depth Measurement
  • 04:57Results: Representative Small Intestine Injury Recovery Assessment
  • 06:17Conclusion

Summary

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Traduction automatique

Here, we present a protocol to establish important endpoints and proliferative markers of small intestinal injury and compensatory hyperproliferation using a model of chemotherapy-induced mucositis. We demonstrate the detection of proliferating cells using a cell cycle specific marker and using small intestinal weight, crypt depth, and villus height as endpoints.

Tags

Keywords: 5-Florauracil5FUChemotherapy-induced MucositisMouse ModelSmall Intestinal InjuryCompensatory HyperproliferationBrdU QuantificationHistological ProcessingImage Analysis

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