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Co farvning blodkar og Nerve fibre i fedtvæv
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Immunologie et infection
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Journal JoVE Immunologie et infection
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Co farvning blodkar og Nerve fibre i fedtvæv

DOI:
10.3791/59266-v

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12:05 min

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February 13, 2019

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, , ,
1Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston, 2Microscopy Core, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston

Chapitres

  • 00:04Titre
  • 00:53Adipose Tissue Collection and Antibody Incubation
  • 05:17Image Acquisition and Analysis of Blood Vessel and Nerve Fiber Networks
  • 09:37Results: Whole Mount Co-immunofluorescence Staining of Blood Vessels and Nerve Fibers in Adipose Tissues
  • 11:17Conclusion

Summary

Traduction automatique

Traduction automatique

Nye blodkar dannes og sympatiske innervation spille centrale roller i fedtvæv remodellering. Der er dog stadig tekniske problemer i visualisering og kvantitativ måling af fedtvæv. Her præsenterer vi en protokol for at med held etiket og kvantitativt sammenligne tætheder af blodkar og nerve fibre i forskellige fedtvæv.

Tags

AngiogenesisSympathetic InnervationBlood VesselsNerve FibersImmunofluorescenceConfocal MicroscopyFixationPermeabilizationBlockingPrimary AntibodiesEndomucinTyrosine Hydroxylase

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