Journal
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Recherche en cancérologie
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Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

DOI:
10.3791/59278-v

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09:17 min

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May 03, 2019

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,
1Cancer Genomics Netherlands,Prinses Máxima Center for Pediatric Oncology

Chapitres

  • 00:04Titre
  • 00:37Surgical Preparation
  • 02:16Tumor Cell Injection and Cranial Imaging Window (CIW) Preparation
  • 03:26Intravital Imaging Setup
  • 04:07Time-Lapse Image Acquisition
  • 05:02Biopsy-Like Injury and CIW Replacement
  • 05:50Image Analysis
  • 06:56Results: Representative Impact of Biopsy on Tumor Cell Migration
  • 08:56Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a method for high-resolution time-lapse multiphoton imaging of brain tumor cells before and after invasive surgical intervention (e.g., biopsy) within the same living animal. This method allows studying the impact of these invasive surgical procedures on tumor cells' migratory, invasive, and proliferative behavior at a single cell level.

Tags

Keywords: Intravital ImagingBrain TumorSurgical BiopsyMouseStereotactic FrameCraniotomyTumor Cell InjectionCranial Imaging Window

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