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Sanntid Live-celle flowcytometri å undersøke kalsium tilstrømningen Pore dannelse og fagocytose av P2X7 reseptorer i voksen nevrale stamceller
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1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne
Chapitres
- 00:04Titre
- 00:58Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
- 02:11Measuring Calcium Influx by Live-cell Flow Cytometry
- 05:21Measuring Pore Formation by Live-cell Flow Cytometry
- 07:02Measuring Phagocytosis by Live-cell Flow Cytometry
- 09:02Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
- 10:46Conclusion
Gir encellede følsomhet, er sanntid flowcytometri unikt tilpasset å kvantifisere flere reseptor funksjoner av levende kulturer. Bruker voksen nevrale stamceller, ble funksjonen P2X7 reseptor vurdert via kalsium tilstrømningen oppdaget av kalsium indikator dye, transmembrane pore dannelsen av ethidium bromide opptak og fagocytose bruker fluorescerende latex perler.
